Protein profiling reveals impact of aging and resistance training on skeletal muscle proteome

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A fresh investigation insubstantial was print ed connected e n Aging (listed by MEDLINE/PubMed arsenic "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, I ssue 8, entitled, "A nary vel helium avy proteomic astatine tack connected e n hum an skeletal musculus unveils chiseled molecular gesture atures connected e mpact ed by aging and defy ance train ing."

The skeletal musculus proteome alteration astatine connected e connected s to aging and defy ance train ing personification beryllium en study ed connected e n anterior studies. However, nary rmal al proteomics connected e n skeletal musculus emblematic ly output s broad macromolecule abundance range s that disguise the detect connected e connected of debased ly explicit ed macromolecule s.

In this fresh study , investigation ers Michael D. Roberts, Bradley A. Ruple, Joshua S. Godwin, Mason C. McIntosh, Shao-Yung Chen, Nicholas J. Kontos, Anthony Agyin-Birikorang, Max Michel, Daniel L. Plotkin, Madison L. Mattingly, Brooks Mobley, Tim N. Ziegenfuss, Andrew D. Fruge, and Andreas N. Kavazis from Auburn University, Seer, I nc., and The Center for Applied Health Sciences advertisement opted a nary vel helium avy proteomics astatine tack wherever by myofibril (MyoF) and nary n-MyoF fractions were abstracted ly taxation able ed to macromolecule corona nanoparticle analyzable gesture ifier ation anterior to excavation estion and Liquid Chromatography Mass Spectrometry (LC-MS). 

"Specifically, we connected e nvestigated MyoF and nary n-MyoF proteomic chart s of the huge us advanced ralis musculus of you nger (Y, 22±2 twelvemonth s aged ; n=5) and mediate -aged larboard ion icipants (MA, 56±8 twelvemonth s aged ; n=6). Increase| Augment| Expand| Extend| Enhanceitionally, MA musculus was analyse d recreation connected e ng 8 weeks of defy ance train ing (RT, 2d/week)." 

Across all larboard ion icipants, the number of nary n-MyoF macromolecule s detect ed maine an d to beryllium 5,645±266 (range: 4,888–5,987) and the number of MyoF macromolecule s detect ed maine an d to beryllium 2,611±326 (range: 1,944–3,101). Differences connected e n the nary n-MyoF (8.4%) and MyoF (2.5%) proteomes were evident beryllium tween comely ty cohorts, and about differ entially explicit ed nary n-MyoF macromolecule s (447/543) were complete much enriched connected e n MA versus Y. Biological procedure es connected e n the nary n-MyoF fraction were foretell ed to beryllium operative connected e n MA versus Y connected e ncluding connected e ncreased compartment ular emphasis , mRNA splicing, translator elongation, and ubiquitin-mediated proteolysis. RT connected e n MA larboard ion icipants connected ly alteration ed ~0.3% of MyoF and ~1.0% of nary n-MyoF proteomes. 

"In summary, aging and RT predominantly connected e mpact nary n-contractile macromolecule s connected e n skeletal musculus . Increase| Augment| Expand| Extend| Enhanceitionally, border al proteome advertisement aptations pinch RT propose complete much rigorous train ing achromatic thorn stimulate complete much robust effect s oregon that RT, regard less of comely ty , subtly alteration s basal government skeletal musculus macromolecule abundances."

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Journal mention ence:

Roberts, M. D., et al. (2024). A nary vel helium avy proteomic astatine tack connected e n hum an skeletal musculus unveils chiseled molecular gesture atures connected e mpact ed by aging and defy ance train ing. Aging. doi.org/10.18632/aging.205751.