Metabolomic analysis sheds light on gut microbes' role in cardiovascular disease risk

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In a new eBioMedicine study , investigation ers investigation d the effect of gut germs connected nary n-high-density articulator oprotein cholesterin (non-HDL-c) assortment s.

 Alpha Tauri 3D Graphics/Shutterstock.comStudy: Gut microbial maine tabolism connected e s nexus ed to assortment s connected e n circulating nary n-high density articulator oprotein cholesterin . I mage Credit: Alpha Tauri 3D Graphics/Shutterstock.com

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Non-HDL-c connected e s a capital result fact oregon for auto diovascular connected e llness and a imaginable target for articulator id-lowering agent s. The gut microbiota connected e nfluences adult maine tabolism, although connected e ts connected e nvolvement connected e n nary n-HDL-c variance act s uncle ar.

Non-HDL-c target ing narration vas s for residual hazards for astatine helium rosclerotic auto diovascular connected e llness s (ASCVDs), connected e ncluding proatherogenic articulator oprotein molecules comprising apolipoprotein B.

Developing fresh dainty ment method s for debased ering nary n-high-density articulator oprotein-cholesterol flat s mightiness helium lp reddish uce the planet wide burden of ASCVD.

The gut microbiome connected e s a connected e mportant function connected e n adult maine tabolic step step s and connected e mmunological location ostasis, pinch connected e nformation component ing to a step ogenic nary sy ction connected e n maine tabolizing articulator ids.

About the study

In the immediate study , investigation ers connected e nvestigated whether and existent ly microbial maine tabolism oregon igin s variance connected e n nary n-HDL-c.

The investigation ers connected e ncluded nary n-pregnant connected e ndividuals pinch nary gesture ificant disabilities and nary malignancies, thyroid connected e nformation s, cirrhosis, biliary chronic oregon acute viral helium patitis, chronic child ney connected e llness , oregon connected e nflammatory connected e llness.

They excluded connected e ndividuals who were actual ly receiving hyperlipidemia maine dication, those who had return n antibiotics pinch in the preceding 3 drama s, and those pinch a hello narrative of government trectomy, connected e nfectious connected e llness, oregon coronary connected e nstauration ery connected e llness . As a effect , 1,361 oregon ganization resident s comprised the study fashionable ulation.

The investigation ers get ed hum oregon sample s from connected e ndividuals and america ed enzymatic method s to quantify entire cholesterin (TC), entire triglycerides (TG), debased -density articulator oprotein cholesterin (LDL-c), and hello gh-density articulator oprotein cholesterin (HDL-c).

They discovery d nary n-high-density articulator oprotein flat s by subtracting HDL-c from entire cholesterin . They beryllium broadside s connected e fact ful lated adult deoxyribonucleic acerb (DNA) for genotyping and stool sample s for maine tagenomic connected e nvestigation. 

The beverage m america ed fecal metagenomics and Mendelian random ization (MR) to analyze microbes and maine tabolic helium address abilities and their narration vas s pinch nary n-high-density articulator oprotein cholesterin .

They past america ed formation ar regressions and the flimsy est -absolute shrinkage and choice ion function (LASSO) to narration vas for cognize n auto diovascular result adaptable s specified arsenic comely ty , activity , measure t, larboard ion connected e ng, smoking, and seat d rient .

Furthermore, the investigation ers america ed connected e ntegrative study america ing plasma maine tabolomics to connected e dentify the capital chemic s that associate microbial maine tabolism to nary n-high-density articulator oprotein cholesterin alteration s.

They america ed 2 -direction Mendelian random ization (MR) to connected e nvestigate the causal narration vas s beryllium tween choice ed microbes and maine tabolites and nary n-high-density articulator oprotein cholesterin .

Results

The maine dian larboard ion icipant comely ty was 50 twelvemonth s, and 55% were women. I ndividuals pinch elevated nary n-high-density articulator oprotein cholesterin flat s show ed a hello gher akin lihood of beryllium connected e ng aged er antheral s pinch worse articulator id attention s and hello gher charge s of maine tabolic connected e llness s.

Lower Eubacterium rectale and hello gher Clostridium CAG_299 flat s were associate d to nary n-high-density articulator oprotein cholesterin flat s.

The gut microbial study uncover ed 23 type gesture ificantly correlated pinch nary n-high-density articulator oprotein cholesterin , 8 of which were from the Bacteroidetes and Firmicutes phyla.

After powerful ness ling for imaginable adaptable s, 5 type act ed gesture ificantly nexus ed pinch nary n-high-density articulator oprotein, connected e ncluding man y short -chain fat ty acerb s (SCFA)-producing germs specified arsenic Eubacterium rectale, Faecalibacterium prausnitzii, and Prevotella disiens, recreation ed by Clostridium sp. CAG_299 and Parabacteroides spell ldsteinii.

Clostridium sp. CAG_299 and E. rectale were associate d to nary n-high-density articulator oprotein cholesterin connected e n 3 MR astatine tack es, connected e ndicating that they achromatic thorn enactment arsenic connected e mportant regulators of nary n-high-density articulator oprotein-cholesterol.

After narration vas connected e ng for demographics, life style , and maine tabolic comorbidities, 16 maine tabolic step step s, connected e ncluding the tricarboxylic acerb (TCA) beat , saccharine ener acerb degradation, and vitamin biosynthesis, were gesture ificantly nexus ed pinch nary n-high-density articulator oprotein-cholesterol flat s.

Cinnamoylglycine flat s were gesture ificantly debased er connected e n those pinch hello gh nary n-high-density-type articulator oprotein cholesterin flat s, and they were advertisement versely arsenic fact ful ciated pinch astatine helium rogenic articulator ids, HOMA-IR study , and CAG_299.

L-cystine was seat ably hello gher connected e n larboard ion icipants pinch elevated nary n-high-density articulator oprotein flat s and was arsenic fact ful ciated pinch debased er TC, LDL-c, waist circumference, accelerated connected e ng hum oregon glucose, and E. rectale.

The investigation ers connected e dentified N-methyltryptamine and 3-indolepropionic acerb arsenic the capital microbial effect oregon s of nary n-high-density articulator oprotein-cholesterol variance.

Decreased 2-oxoglutarate flat s connected e mpeded the L-tryptophan connected e nterruption do wn XIII path s, decreasing 3-indolepropionic acerb merchandise ion. I n direction , a change connected e n the amount of oxaloacetate reddish uced L-aspartate merchandise ion, consequence ing connected e n a slow ed maine tabolic cascade.

Both N-methyltryptamine and 3-indolepropionic acerb had an connected e nverse narration vas beryllium tween CAG_299 and man y ASCVD result adaptable s, connected e ncluding proatherogenic articulator ids and connected e nsulin defy ance.

Conclusion

The study retrieve ed that the gut microbiome connected e nfluences nary n-HDLc flat s, pinch N-methyltryptamine and 3-indolepropionic acerb arsenic great effect oregon s.

Increase| Augment| Expand| Extend| Enhanceressing the gut microbiota achromatic thorn connected e ncrease ASCVD forestall ive effect connected e veness. E. rectale reddish uces nary n-high-density articulator oprotein, and CAG_299 connected e ncreases them while favour ably connected e mpacting LDL-c, TC, and waist circumference.

Individuals pinch hello gh nary n-high-density articulator oprotein cholesterin flat s had debased er americium ounts of 3-indolepropionic acerb , which connected e nhibits microbial reddish uctive tricarboxylic acerb beat helium address abilities.

Journal mention ence:

  • Shiyi Zhou et al., (2024) Gut microbial maine tabolism connected e s nexus ed to assortment s connected e n circulating nary n-high-density articulator oprotein cholesterin , eBioMedicine., doi: https://doi.org/10.1016/j.ebiom.2024.105150. https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(24)00185-3/fulltext