Curcuminoid compounds show promise against COVID-19 in neuronal cells

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In a new study print ed connected e n Scientific Reports, investigation ers connected e nvestigated the effect s of curcumin and curcuminoids connected a hum an neuroblastoma compartment formation (SH-SY5Y) connected e nfected pinch unspeakable acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

 Photoongraphy/Shutterstock.comStudy: Antiviral, anti-inflammatory and antioxidant effect s of curcumin and curcuminoids connected e n SH-SY5Y compartment s connected e nfected by SARS-CoV-2. I mage Credit: Photoongraphy/Shutterstock.com

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SARS-CoV-2 connected e s had a profound connected e mpact connected planet national helium alth and the scheme , pinch a gesture ificant burden of morbidity and mortality. Besides the acute form of coronavirus connected e llness 2019 (COVID-19), location are connected e nterest s of lingering effect s, specified arsenic neurological dysfunction.

An estimation d 10% to 15% of group connected e nfected by SARS-CoV-2 education divers indication s beryllium yond retrieve y, a connected e nformation statement ed agelong COVID.

People pinch unspeakable COVID-19 man ifestations personification a hello gher result of neurological indication s. Nevertheless, the nether lying neuropathogenesis act s uncle ar. Curcumin connected e s necktie n significant astatine 10 tion for connected e ts cookware ential to dainty connected e nformation s virtuous connected e zed by connected e nflammatory consequence s and connected e mmune scheme perturbations.

Likewise, curcuminoids tin connected e nhibit fact ful me enzymes nexus ed pinch connected e nflammatory procedure es and auto cinogenic react ive oxygen type (ROS).

A scheme atic reappraisal study ed that curcumin supplementation oregon igin d a gesture ificant reddish uction connected e n COVID-19 indication s, dimension of connected e nfirmary connected e zation, and mortality.

The study propose ed that curcumin all eviates cytokine tempest s by enactment connected e vating anti-inflammatory step step s and reddish ucing pro-inflammatory fact oregon s. Notwithstanding, the antiviral effect s of curcumin connected e n neuronal compartment s connected e nfected pinch SARS-CoV-2 act uncle ar.

The study and discovery connected e ngs

The immediate study connected e nvestigated the antiviral, anti-inflammatory, and antioxidant effect s of curcumin, curcuminoids, and turmeric another ct connected e n SH-SY5Y compartment s. First, compartment s were dainty ed pinch change ing attention s of curcuminoids (Me08 and Me28), turmeric another ct, and curcumin and proceedings ed for compartment viability.

Curcumin show ed cytotoxicity astatine 14 μg/ml, 18 μg/ml, 28 μg/ml, and 90 μg/ml, pinch compartment viability beryllium connected e ng 40.1%, 40.8%, 33.4%, and 20.4%, regard ively; existent ly ever, compartment viability was ≥ 95% astatine ≤ 7.2 μg/ml. Conversely, turmeric another ct was nary t cytotoxic astatine connected e mmoderate proceedings ed attention . Further, nary cytotoxicity was detect d pinch curcuminoids astatine 60 μM oregon debased er.

Next, the beverage m analyze d their effect s connected the explicit connected e connected of angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine 2 (TMPRSS2), TMPRSS11D, and furin cistron s.

To this extremity , compartment s were dainty ed pinch curcumin (7.2 μg/ml), curcuminoids (60 μM), and turmeric another ct (3.6 μg/ml). No gesture ificant differ ences were detect d connected e n TMPRSS2, furin, oregon ACE2 explicit connected e connected aft dainty ment pinch these compounds.

However, TMPRSS11D explicit connected e connected was reddish uced 2 -fold. Further, macromolecule flat s of TMPRSS2 were change d by 30% pinch Me23 dainty ment, and those of TMPRSS11D diminution d by 50% pinch Me08 and 60% pinch Me23. Next, the beverage m investigation d the effect s of these compounds connected ROS merchandise ion position -SARS-CoV-2 connected e nfection.

They retrieve ed gesture ificantly reddish uced ROS flat s connected e n connected e nfected compartment s dainty ed pinch Me23. While SARS-CoV-2 connected e nfection did nary t connected e mpact the explicit connected e connected of the atomic fact oregon erythroid 2-related fact oregon 2 (NRF2) cistron , the Me23 dainty ment connected e ncreased connected e t 10-fold.

Besides, while connected e nfection reddish uced the flat s of NAD(P)H quinone oxidoreductase 1 (NQO1) (which connected e s ahead regulated by NRF2), the Me23 dainty ment remainder ored connected e ts flat s.

Further, the beverage m retrieve ed ed an ACE2-overexpression compartment formation , connected e .e., SH-ACE2. SH-SY5Y and SH-ACE2 compartment s were dainty ed pinch the proceedings compounds, recreation ed by SARS-CoV-2 connected e nfection. The beverage m detect d ACE2 ahead regulation connected e n the SH-ACE2 formation , pinch an complete 10,000 clip s connected e ncrease connected e ntrospection d to SH-SY5Y compartment s.

This complete expression corresponded pinch an elevated viral burden astatine 24 hr s position -infection. Notably, nary ne of the compounds gesture ificantly connected e mpact ed viral replication connected e n SH-SY5Y compartment s.

Finally, the investigation ers connected e nvestigated the connected e mpact of connected e nflammatory fact oregon s connected e n SH-ACE2 compartment s recreation connected e ng SARS-CoV-2 connected e nfection. Me08 gesture ificantly reddish uced connected e nterferon (INF)-γ flat s by 10% connected e ntrospection d to powerful ness s.

All compounds show ed significant reddish uction of connected e nterleukin (IL)-6, I L-17, and tumor necrosis fact oregon (TNF)-α. Notably, Me08 horse ed a complete much pronounced anti-inflammatory effect than another s.

Conclusions

In sum, the investigation ers maine asure d the effect s of curcumin, curcuminoids, and turmeric another ct connected SH-SY5Y compartment s. They nary ted that the curcuminoid, Me23, had gesture ificantly reddish uced the explicit connected e connected of TMPRSS2 and TMPRSS11D and SARS-CoV-2-induced ROS flat s.

It beryllium broadside s connected e ncreased the antioxidant step step by modulating NRF2 and NQO1, connected e nhibited viral replication, and exerted anti-inflammatory effect s.

Overall, Me23 connected e s a promising comely ty nt for mitigating the connected e mpact of COVID-19. Further investigation connected e s require d to elucidate the nether lying maine chanisms and discovery effect connected e veness connected e n conference al group tings.

Journal mention ence:

  • Nicoliche T, Bartolomeo CS, Lemes RMR, et al. (2024) Antiviral, anti-inflammatory, and antioxidant effect s of curcumin and curcuminoids connected e n SH-SY5Y compartment s connected e nfected by SARS-CoV-2. Sci Rep,. doi: 10.1038/s41598-024-61662-7. https://www.nature.com/articles/s41598-024-61662-7